L. Chitradon et al., Oligosaccharide synthesis by reversed catalysis using alpha-amylase from Bacillus licheniformis, J MOL CAT B, 10(1-3), 2000, pp. 273-280
Evidence is provided for the synthesis of hetero-oligosaccharides by Termam
yl, the thermotolerant ol-amylase from Bacillus lichenifomis. In the presen
ce of soluble starch and added non-starch sugars (isomaltose, cellobiose, m
annose, melibiose, stachyose, xylose and 1-o-methyl-beta-D-glucopyranoside)
, oligosaccharides were produced by the reversed catalytic reaction. New pe
aks of homo- and hetero-oligosaccharides (DP1-10) were detected by high per
formance anion exchange chromatography (HPAEC) in equilibrium reaction mixt
ures. Attempts to increase the extent of synthesis of oligosaccharides usin
g organic solvents (ethanol, methanol, N-propanol, propanediol, N-butanol,
dioxane and dimethyl sulfoxide (DMSO)) to lower the water activity of the r
eaction mixture were unsuccessful. Raising the temperature of the reaction
mixture from 37 degrees C to 55 degrees C was ineffective. (C) 2000 Elsevie
r Science B.V. All rights reserved.