Cm. Cheffings et D. Colquhoun, Single channel analysis of a novel NMDA channel from Xenopus oocytes expressing recombinant NR1a, NR2A and NR2D subunits, J PHYSL LON, 526(3), 2000, pp. 481-491
1. Two types of subunit (an NR1 subunit and one of the NR2 subunits) are su
fficient to form efficient NMDA receptors. In order to investigate whether
functional receptors mag contain more than one sort of NR2 subunit, NR1, NR
2A and NR2D subunits were co-expressed in Xenopusoocytes.
2. Single channel recordings from the oocytes showed, as expected, channel
openings that are characteristic of NR1/NR2A receptors (ca 50 and 40 pS con
ductances), and channels characteristic of NR1/NR2D receptors (ca 40 and 20
pS conductances). However, they also contained a novel channel that contai
ned conductances of ca 30, 40 and 50 pS, with direct transitions between al
l three levels.
3. It is postulated that the novel channel contains NR1, NR2A and NR2D subu
nits. The implications of this for receptor stoichiometry are discussed.
4. The novel channel was intermediate between the NR1/NR2A and NR1/NR2D 'du
plet' receptors in the length of the channel activations, and in the probab
ility of being open during activation. Its glycine sensitivity was much hig
her than that of NR1/NR2A, and was comparable with that of the other 'duple
t' receptors.