D. Mares et al., Effectiveness of four new pyrazole-pyrimidines on phytopathogens: Ultrastructural evidences on Pythium ultimum, J PHYTOPATH, 148(7-8), 2000, pp. 395-403
Citations number
15
Categorie Soggetti
Plant Sciences
Journal title
JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT
Four newly synthesized molecules derived from pyrazole-pyrimidine were assa
yed on Botrytis cinerea Micheli, Fusarium moniliforme Sheld and Pythium ult
imuun Trow. All proved effective in inhibiting the growth of the phytopatho
gens at all of the test concentrations (10, 20, 50, 100 mu g/ml). The most
effective compound was 1-(3)nitrophenyl-6-trifluoromethylpyrazolo[3,4-d]pyr
imidine-4(5H)-thione (CF(3)3). Ultrastructural studies on P. ultimum treate
d with CF(3)3 revealed alterations in the normal hyphal shape and, at high
concentration, plasmolysis and damage to the wall texture was observed. At
20 mu g/ml different vesicles were seen in the cytoplasm: some appeared qui
te dense, and specific cytochemical reactions indicated that they were most
likely peroxysomes; other vesicles seem to be vacuoles of varying content.
In some cases there was disintegration of the nuclear envelope. The effect
s on membrane lipids and interference in protein synthesis are hypothesized
as possible mechanism of action of the molecule.