Since enterohemorrhagic Escherichia coli O157, Salmonella, etc., sometimes
contaminate animal feces and may cause infectious diseases to humans, it is
important to remove pathogenic bacteria from domestic animal waste. For th
e purpose, we examined the antibacterial activity of chaff vinegar. We foun
d that the chaff vinegar inhibited the growth of pathogenic bacteria immedi
ately in vitro but not efficiently spores and lactic acid bacteria. Further
, it removes bacteria, especially Enterobacteriaceae, from animal feces and
the surface of the concrete-floor in the cattle barn. Chaff vinegar is adv
ertised as. natural chemical substance for a soil conditioner, to promote t
he composting and to deodorize their smell. Chaff vinegar may be useful for
organic agriculture without enteric pathogenic bacteria.