Hydrophilic/hydrophobic patterned surfaces as templates for DNA arrays

Authors
Gillmor, SD Thiel, AJ Strother, TC Smith, LM Lagally, MG
Citation
Sd. Gillmor et al., Hydrophilic/hydrophobic patterned surfaces as templates for DNA arrays, LANGMUIR, 16(18), 2000, pp. 7223-7228
Citations number
16
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
LANGMUIR
ISSN journal
07437463 → ACNP
Volume
16
Issue
18
Year of publication
2000
Pages
7223 - 7228
Database
ISI
SICI code
0743-7463(20000905)16:18<7223:HPSATF>2.0.ZU;2-J
Abstract
Addressable DNA arrays are created by photopatterning self-assembled monola yers to form hydrophilic and hydrophobic regions on a gold surface. The hyd rophilic regions aid to contain small volumes of different DNA solutions pl aced on them using an automated pin-tool loading strategy. The method allow s for efficient attachment, manipulation, and hybridization of pure DNA str ands on the surface.