Examination of the effect of repeated blood takings on carbohydrate metabolism in rabbits with the determination of glycated proteins and blood glucose level

In domestic animals several blood parameters are measured in order to help the clinical diagnosis and to monitor the metabolic state of the animal. Blood glucose (blood sugar) is one of the exact parameters that determines the carbohydrate/energy metabolism In the domestic animals and in humans. T his parameter (if determined only in itself) is not always reliable, becaus e some changes e.g. sympathoadrenal activation may alter its actual value. The effect of some pharmacons on certain blood parameters of the rabbits (n =5-5) was investigated in the experiment. After taking the initial blood (p robe 0) 1 ml/bwkg sterile physiological NaCl solution was injected i.p. in the first, so called control group. The other, so called experimental group s were given the following pharmacons also in the form of i.p. injections: 1 ml/bwkg (1:10 diluted ampulla) atropin, 50 mu g/bwkg tonogen, 50 mu g/bwk g acetylcholin, and 1 NE/bwkg insulin. From repeated blood takings in the 5 ., 10., 20., 30., 60., 120., and 240. minutes after injection the following parameters were investigated: hematocrit, hemoglobin, blood plasma glucose , total protein, albumin, albumin %, globulin, IgG; as well as the glycated blood proteins (glycated hemoglobin, GHb and serum/plasma fructosamine, Se Fa). It was concluded, that on the effect of repeated blood takings the glucose concentration even of the control groups increased significantly, while the level in glycated proteins, especially of the glycated hemoglobin changed only very little. So, the latter ones proved to be better markers for the l ong term, more certain determinations of blood glucose levels. That is why the determination of all the three parameters (blood plasma glucose, SeFa a nd GHb) increases the reliability of the analysis of carbohydrate metabolis m in rabbits.