Influence of water polluted with herbicide Roundap on the organism of fish

Roundup, also known by the name Glyphosate, is used as a non-selective herb icide in agriculture as well as in fish ponds, lakes, canals etc. Literatur e on the toxicity of glyphosate for the aquatic environment and aquatic org anisms is extremely scarce. The objective of this study was to investigate the effects of different sublethal concentrations of glyphosate (4, 6, 8 mg /l) in water on the non-specific immunity and the following internal organs : livers and kidneys of carps. The study was carried out on 120 clinically healthy carps K-1, weighing 60-80 g. The white blood cell count (WBC), neut rophil count and micro test of NBT reduction to evaluate phagocytic activit y of neutrophils were performed. Likewise the phagocytic activity of kidney neutrophils and macrophages as well as the level of lysozyme in serum were observed. During all the experiments (28 days) one observed decreased non- specific immunity. The reduction of the phagocytic activity of blood and ki dney neutrophils and macrophages as well as of the lysozyme level was obser ved. In the histopathological investigations degenerative changes in the livers and kidneys of the carps exposed to glyphosate during 14 days were observed . Histopathological changes in livers were characterized with oedema cells, vacuolar degeneration and focal fibrosis. In kidneys, degenerative changes relating mostly to tubular cells were noted, as well as necrosis of heamop oietic tissue and focal fibrosis. The studies showed that Roundup in toxic to fish, handicapping the functioning of the defensive immunological system and liver and kidney function.