Microsatellite DNA variants between the inbred SENCAR mouse strains

The two-stage model, initiation with 7,12-dimethylbenz[a]anthracene and pro motion with 12-O-tetradecanoylphorbol-13-acetate, of mouse skin carcinogene sis has been the protocol of choice to study the genetic susceptibility to carcinogens, the outbred SENCAR mouse being the most widely used skin tumor -sensitive animal model. Squamous cell carcinomas (SCCs) develop from many of the papillomas, making these mice a useful model for epithelial tumorige nesis and for the progression to malignant tumors. Nine different inbred st rains derived from outbred SENCAR mice have been recently reported. Interes tingly, these strains display different sensitivities to two-stage carcinog enesis, and, in particular, some of them show a dissociation between suscep tibility to papilloma development and the malignant conversion of these int o SCC. However, the utility of these SENCAR strains for genetic mapping is limited by the lack of information regarding DNA variant alleles among them . Therefore, we analyzed the nine inbred strains with microsatellite marker s distributed along the 20 chromosomes and in this article report the varia nt alleles found. The information presented is likely to be helpful for lin kage analysis and marker-assisted development of congenic strains between S ENCAR-derived inbred strains. (C) 2000 Wiley-Liss, inc.