Genotoxicity evaluation of heat shock in gold fish (Carassius auratus)

Genotoxicity evaluation of heat shock was carried out in Carassius auratus. The genotoxicity end points studied were nuclear anomalies (micronucleus a ssay), chromosomal aberrations, DNA damage (comet assay) and cell prolifera tion. The heat shock temperatures used were 34 degrees C, 36 degrees C and 38 degrees C. The results demonstrated that heat shock causes the induction of micronucleus at all the three temperature studied. Heat shock also inhi bited cell proliferation at 38 degrees C acid caused aberrations in the met aphase chromosomes at 34 degrees C and 36 degrees C. Comet assay demonstrat ed single strand DNA damage at all the three temperatures. The results obta ined indicate that heat shock is a genotoxicant. (C) 2000 Elsevier Science B.V. All rights reserved.