Invasion of strongly binding oligonucleotides into tRNA structure

Authors
Petyuk, V Serikov, R Tolstikov, V Potapov, V Giege, R Zenkova, M Vlassov, V
Citation
V. Petyuk et al., Invasion of strongly binding oligonucleotides into tRNA structure, NUCLEOS NUC, 19(7), 2000, pp. 1145-1158
Citations number
24
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
19
Issue
7
Year of publication
2000
Pages
1145 - 1158
Database
ISI
SICI code
1525-7770(2000)19:7<1145:IOSBOI>2.0.ZU;2-0
Abstract
Interaction of yeast tRNA(Phe) with oligodeoxyribonucleotides containing 5- methylcytosine, 2-aminoadenine, and 5-propynyl-2'-deoxyuridine was investig ated. The modified oligonucleotides show increased binding capacity althoug h the association rates are similar for the modified and natural oligonucle otides. The most pronounced increase in association constant (70 times) due to the incorporation of the strongly binding units was achieved in the cas e of oligonucleotide complementary to the sequence 65-76 of the tRNA(Phe).