Humoral responses to a secretory Onchocerca volvulus protein: differences in the pattern of antibody isotypes to recombinant Ov20/OvS1 in generalizedand hyperreactive onchocerciasis
Jl. Mpagi et al., Humoral responses to a secretory Onchocerca volvulus protein: differences in the pattern of antibody isotypes to recombinant Ov20/OvS1 in generalizedand hyperreactive onchocerciasis, PARASITE IM, 22(9), 2000, pp. 455-460
The Onchocerca volvulus secretory protein Ov20/OvS1 represents a dominant a
ntigen expressed in the infective larvae, microfilariae and adult stages of
the parasite. The humoral responses to this protein have not yet been anal
ysed in the polar clinical and immunological forms of onchocerciasis. Analy
sis by ELISA of class and subclass antibodies to Ov20/OvS1 in per-sons with
the generalized or the hyperreactive form of onchocerciasis revealed simil
ar strong responses of IgG1, IgG4 and IgM antibody levels in both forms of
onchocerciasis and significant differences were observed in the IgE and IgA
antibody classes. Computation of the ratios of antibodies showed that pers
ons with the generalized form exhibited significantly higher ratios of IgG4
to IgG1, IgG4 to IgE, and IgM to IgE than patients with the hyperreactive
form,. To investigate the isotype recognition of antigenic sites oil Ov20/O
vS1 protein, three recombinantly expressed fragments (F1-3) of Ov20/OvS1 we
re probed rising sera which strongly reacted with intact recombinant Ov20/O
vS1. Epitope(s) on F1 comprising amino acid residues 1-63 were significantl
y recognized by IgG1 min IgE, while IgM recognized epitopes on all three fr
agments. The strongest reaction of IgM occurred with epitope(s) formed by r
esidues 105-171 (F3). In contrast, IgG4 type antibodies were not reactive w
ith either of the three OvS1 fragments, but they reacted with intact Ov20/O
vS1 protein. Generalized onchocerciasis, unable to eliminate microfilariae,
and hyperreactive onchocerciasis, with a high potency to eliminate or to r
educe parasite loads, can be distinguished by a distinct pattern of isotype
responses to Ov20/OvS1.