Ams. Pereira et al., Co-cultivation of plant cells as a technique for the elicitation of secondary metabolite production, PL CELL TIS, 60(3), 2000, pp. 165-169
Cells of Mikania glomerata, Cephaelis ipecacuanha and Maytenus aquifolia we
re co-cultured in a two-phase system using filter paper as a solid support.
The species were co-cultured in all possible paired combinations. Interact
ion between Mikania and Maytenus cells resulted in increased biomass produc
tion of Maytenus cells, but the friedelin content was reduced. Co-cultivati
on of Cephaelis and Mikania cells enhanced coumarin content, but inhibited
the growth of Mikania cells. However, yield of emetine as well as Cephaelis
biomass accumulation were positively stimulated by the co-cultivation. Res
ults indicate a possible occurrence of allelopathy in such a system.