Plant gene silencing was originally thought to be a quirk of transformation
procedures, but is now recognized to be a facet of vitally important gene
regulatory systems, present in all organisms. Monocot plants, especially th
e grasses, play a foremost role in the agricultural economy of all nations,
and their biotechnological manipulation offers great potential for both de
veloped and developing countries. Here, we review reported instances of tra
nsgene silencing in monocots and relate the processes of transcriptional an
d post-transcriptional gene silencing (TGS, PTGS) in perspective to the rap
idly burgeoning knowledge of these phenomena in many organisms. Recent find
ings include the involvement of an RNA-dependent RNA polymerase and a nucle
ase in PTGS systems and the close relationship between methylation and chro
matin structure in TGS events.