A new tool for superoxide and nitric oxide radicals determination using suitable enzymatic sensors

A new tool for radical determination is investigated. Superoxide and nitric oxide radicals have been determined using their modulating action on three different enzymatic sensors based on tyrosinase, superoxide dismutase (SOD ), and galactose oxidase (GAO), respectively. Basic research was carried nu t to study the inhibition or activation effect of the two radicals consider ed on the enzyme sensor's response and experimentally evidencing the correl ation between the modulating action of the biosensor response and the radic al concentration in solution. Results are shown as linear ranges, which dif fer according to the biosensor used and the radical determined. (C) 2000 El sevier Science S.A. All rights reserved.