Genomic and pathogenic studies on a glycoprotein E variant field isolate of bovine herpesvirus 1

Glycoprotein E-negative (gE-) laboratory strains of bovine herpesvirus 1 (B HV-1) were recently introduced as novel marker vaccines, allowing serologic al discrimination between vaccinated and naturally infected animals on the basis of lack or presence of antibodies against gE epitopes. The applicabil ity of this approach is based on the genetic stability of the gE. However, mutant field variants of BHV-1 with a variable response in anti-gE ELISA ha ve been isolated. The molecular characterization of a gE variant field isol ate (Salwa strain) is presented here. By comparing the gE nucleotide and am ino acid sequences of the Salwa strain with those of the wild strain Jura, ten mutated bases were found in the gE strain of Salwa, six of which alter the amino acid sequence, leading to changes in five amino acids. Both strai ns caused respiratory disease in experimentally infected calves, but Salwa generated slightly milder signs. Both viruses were excreted in nasal and oc ular discharges, and were reactivated by dexamethasone treatment. In conclu sion, the rather close similarities observed in the gE gene structure and p athogenicity features of the gE mutant and of the wild strain of BHV-1 conf irm the genetic stability of gE. The findings indicate that the Salwa isola te is virulent, but less virulent than wild strains. Our data support the u se of gE-negative marker vaccines in eradication programmes.