New derivative of beta-cyclodextrin as chiral selectors for the capillary electrophoretic separation of chiral drugs

The uncharged beta-cyclodextrin (beta-CD) derivative mono-3-O-phenylcarbamo yl-beta-CD was successfully used as chiral selector in capillary zone elect rophoresis. Several chiral drugs such as propafenone and verapamil enantiom ers belonging to different classes of compounds of pharmaceutical interest were resolved. The chiral resolution (Rs) was strongly influenced by the co ncentrations of the cyclodextrin derivative, the background electrolyte, an d the pH of the background electrolyte. The influence of organic modifier w as also studied. Under the conditions of 40 mmol/l berate buffer at pH 9.18 containing 2.5 mmol/l mono-3-phenylcarbamoyl-beta-CD, baseline separation (Rs=3.88 for verapamil, Rs=3.32 for propafenone) of the enantiomers can be achieved. (C) 2000 Elsevier Science B.V. All rights reserved.