Effects of chinonin on nitric oxide free radical, myocardial damage and arrhythmia in ischemic-reperfusion injury in vivo

With sodium nitrite NaNO2 as a standard source of nitric oxide, we compared the correlation coefficients obtained by three measuring methods used curr ently in the determination of the NOFe2+(DETC)(2) complex (DETC, N,N-diethy ldithiocarbamate) with that of the measuring method suggested in this study . The result showed that measuring the total height of triplet signals was the best linear correlation to the concentration of NO compared with other methods used in this system. With this method, we observed the effect of ch inonin on the NOFe2+(DETC), complex in myocardial ischemic-reperfusion inju ry in vivo. The hearts of Wistar rats were subjected to 30 min of ischemia and 10 min of reperfusion in vivo. Different doses of chinonin (5, 10, 25, 50 mg/kg intraperitoneally) were administered to the ischemic-reperfusion r ats. Chinonin increased the signal intensity of the NOFe2+(DETC)(2) complex , inhibited the formation of thiobarbituric acid reaction substance and rel ease of creatine kinase, and mitigated the incidence of ventricular arrhyth mia in a dose-dependent way. Chinonin has cardiovascular protective effects by means of adjusting the level of NO and inhibiting oxygen free-radical-i nduced lipid peroxidation in myocardial ischemic-reperfusion injury in vivo .