W. Koedprang et U. Na-nakorn, Preliminary study on performance of triploid Thai silver barb, Puntius gonionotus, AQUACULTURE, 190(3-4), 2000, pp. 211-221
The triploid chromosome condition was induced in Thai silver barb (Puntius
gonionotus) by application of cold shock (2 degrees C) to eggs at time inte
rvals after activation of 0.5 min with a duration of 10 min which resulted
in mean triploidy yield of 72.5% at 9 months of age. Growth rate of the 2-9
-month-old, cold shock group (0.1-6.2 g/month) did not differ from that of
the control (0.1-5.7 g/month). Gonadal somatic indices (GSI) of presumed tr
iploid males and females were lower than that of control (GSI values of the
presumed triploids were 35.0-60.2% and 28.7-75.9% of control males and fem
ales, respectively). Spermatogenesis and oogenesis were retarded in triploi
ds. However, all stages of spermatogenic cells were observed in triploid ma
les, including few spermatozoa. Oocytes of triploid females did not undergo
vitellogenesis while normal oogenesis was observed in diploids. Nuclear vo
lume of red blood cells (RBCs) of triploid fish was 1.63 times larger than
that of diploids. (C) 2000 Elsevier Science B.V. All rights reserved.