Maturation of human monocyte-derived dendritic cells studied by microarrayhybridization

We compared the transcript profiles of human myeloid immature dendritic (ID C) cells and mature dendritic cells (MDC) by hybridization of cell-derived cDNA to DNA probes immobilized on microarrays. The microarrays contained pr obes for 4110 known genes. We report maturation-dependent changes in transc ription of clusters of differentiation, cytokines, cytokine receptors, chem okines, chemokine receptors, neuropeptides, adhesion molecules, and other g enes. We identified 1124 transcripts expressed in IDC and 1556 transcripts expressed in MDC. Maturation increased the levels of 291 transcripts twofol d or more and reduced the levels of 78 transcripts to one-half or less than in IDC. We identified a concerted maturation-stage-dependent transcription of the variable chains of the members of the gamma-chain-cytokine receptor family IL-4R, IL-7R, and IL-15R. Also, we found the reversal of the ratio of transcripts for galectin-3 and galectin-9 upon maturation. We identified maturation-dependent changes in the levels of transcripts for numerous gen es encoding proteins previously undetected in dendritic cells such as indol eamine 2,3-deoxygenase, Epstein-Barr virus induced protein 3 and kinesin-a. Moreover, MDC transcribed and translated insulin like growth factor-1 rece ptor, transforming growth factor alpha, and neuropeptide Y. (C) 2000 Academ ic Press.