T. Nishio et al., Molecular identification of a rat novel organic anion transporter moat1, which transports prostaglandin D-2, leuko-triene C-4, and taurocholate, BIOC BIOP R, 275(3), 2000, pp. 831-838
Citations number
42
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
We have isolated a rat novel multispecific organic anion transporter, moat1
, The isolated clones were originated by alternative splicing of the moat1
mRNA. The nucleotide sequences predict a protein of 682 amino acids with mo
derate sequence similarity to LST-1, the oatp family, and the prostaglandin
transporter. Northern blot analysis of rat moat1 identified a predominant
transcript of 4.4 kilonucleotides in all tissues. Northern blot and in situ
hybridization analyses of rat brain further indicated that moat1 mRNA is w
idely distributed in neuronal cells of the central nervous system, especial
ly in the hippocampus and cerebellum. moat1 transports prostaglandin D-2 (K
-m; 35.5 nM), leukotriene C-4 (K-m; 3.2 mu M) and taurocholate (K-m; 17.6 m
u M) in a sodium-independent manner, moat1 also transports prostaglandin E-
1, E-2, thromboxane B-2, and iloprost but not dehydroepiandrosterone sulfat
e and digoxin, of which the substrate specificity is similar, but definitiv
ely different from those of any other organic anion transporters, (C) 2000
Academic Press.