Dielectric relaxation measurements of 12 kbp plasmid DNA

Authors
Bakewell, DJ Ermolina, I Morgan, H Milner, J Feldman, Y
Citation
Dj. Bakewell et al., Dielectric relaxation measurements of 12 kbp plasmid DNA, BBA-GENE ST, 1493(1-2), 2000, pp. 151-158
Citations number
44
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
ISSN journal
01674781 → ACNP
Volume
1493
Issue
1-2
Year of publication
2000
Pages
151 - 158
Database
ISI
SICI code
0167-4781(20000907)1493:1-2<151:DRMO1K>2.0.ZU;2-8
Abstract
The dielectric properties of 12 kbp plasmid DNA have been measured as a fun ction of temperature in the range 5 degrees C to 40 degrees C. Time domain reflectometry was used to obtain dielectric data over the frequency range f rom 200 kHz to 3 GHz. Values of the frequency dependent polarisability per DNA macromolecule have been determined from the measurements. Possible mech anisms that could account for the dielectric dispersion are also discussed, in particular the counterion fluctuation model of Manning-Mandel-Oosawa. ( C) 2000 Elsevier Science B.V. All rights reserved.