Protein bodies in the pine megagametophyte in vitro culture: ultrastructural, histochemical and electrophoretic observations

Authors
Hrib, J Janisch, R Vookova, B Nahalkova, J Gemeiner, P Hajduch, M
Citation
J. Hrib et al., Protein bodies in the pine megagametophyte in vitro culture: ultrastructural, histochemical and electrophoretic observations, BIOL PLANT, 43(3), 2000, pp. 329-336
Citations number
22
Categorie Soggetti
Plant Sciences
Journal title
BIOLOGIA PLANTARUM
ISSN journal
00063134 → ACNP
Volume
43
Issue
3
Year of publication
2000
Pages
329 - 336
Database
ISI
SICI code
0006-3134(2000)43:3<329:PBITPM>2.0.ZU;2-S
Abstract
The megagametophytes of the European black pine (Pinus nigra Am.) were cult ured on modified MS medium. After 10 d, protein bodies showed well-marked d egradation on freeze-etched replicas and in preparations observed by scanni ng electron microscopy. After 20 d of cultivation, the megagametophyte cell s were completely empty. Proteins secreted into the agar medium were determ ined by electrophoresis and 15 different proteins, in the range of 6.5 to 7 1 kDa, were identified.