Scale-up of hydrophobic interaction chromatography for the purification ofa DNA vaccine against rabies

Authors
Diogo, MM Ribeiro, S Queiroz, JA Monteiro, GA Perrin, P Tordo, N Prazeres, DMF
Citation
Mm. Diogo et al., Scale-up of hydrophobic interaction chromatography for the purification ofa DNA vaccine against rabies, BIOTECH LET, 22(17), 2000, pp. 1397-1400
Citations number
9
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
17
Year of publication
2000
Pages
1397 - 1400
Database
ISI
SICI code
0141-5492(200009)22:17<1397:SOHICF>2.0.ZU;2-B
Abstract
The purification of a DNA vaccine against rabies by hydrophobic interaction chromatography (HIC) using a Sepharose gel derivatised with 1,4-butanediol diglycidyl ether was scaled up 60 times. The purification profile was not affected by increased loadings (up to 15 mg DNA) and a product with a consi stent quality was obtained. Fourteen mg of plasmid with an HPLC purity of 1 00% were obtained in one run, corresponding to a 95% yield.