Phytochelatins (PCs, (gamma Glu-Cys)(n)-Gly, n = 2-11) are metal ions-bindi
ng peptides produced by plant, algae and fungi. Antibodies that recognize P
Cs were induced by the injection of Balb/c mice with a multiple antigen pep
tide consisting of PC6 (MAP-PC6). One stable hybridoma producing a monoclon
al antibody (mAb), designated as 4-9C, was established. The DNA sequences o
f the heavy and light chain variable regions of the 4-9C mAb were determine
d. The 4-9C mAb had a smaller equilibrium dissociation constant (K-d) towar
ds Cu-, Zn- and Ni-PC7 complexes than those towards other metal-PC7 complex
es and free PCs.