Morphometric measures from 1995-1998 were used to develop a discriminant fu
nction that provides investigators with a practical, non-destructive techni
que for sexing American Bitterns (Botaurus lentiginosus). Thirty-two males
were lured into mirror traps and mist nets using tape-recorded territorial
vocalizations and 17 females were captured at nest-sites using long-handled
dip nets. Sex of captured birds was known because only males respond aggre
ssively to territorial vocalizations and only females incubate nests. Avera
ge morphometric measures were greater for male than female American Bittern
s with overlap between the sexes. Tarsus length was the single most useful
measurement in discriminating between sexes, correctly identifying 100% of
individuals used to construct the function and 71.4% of birds that were not
used in model development (hold-out test data set). The addition of short
bill length measurements increased the proportion of correctly classified i
ndividuals in the hold-out test data set to 76.2% for males and 85.7% for f
emales. This technique will enable field ecologists to separate population
and behavioral data according to sex.