Citation
Wq. Luo et al., Cloning and expression of a novel human HCUTA cDNA, CHIN SCI B, 45(14), 2000, pp. 1301-1304
Citations number
11
Categorie Soggetti
Multidisciplinary
Journal title
CHINESE SCIENCE BULLETIN
ISSN journal
10016538
→ ACNP
Volume
45
Issue
14
Year of publication
2000
Pages
1301 - 1304
Database
ISI
SICI code
1001-6538(200007)45:14<1301:CAEOAN>2.0.ZU;2-B
Abstract
Copper is one of the most important trace elements to life. Human HCUTA is
a novel cDNA encoding a 156aa protein, which may participate in human coppe
r tolerance system. The HCUTA protein is highly similar to protein CUT A1 o
f E. coli. The whole opening reading frame of HCUTA cDNA was amplified by P
CR and cloned into pET28a + express vector, and the HCUTA protein was effec
tively expressed in E. coli BL21 (DE3).