THE MOST PREVALENT PROTEIN IN A HEAT-TREATED EXTRACT OF PEA (PISUM-SATIVUM) EMBRYOS IS AN LEA GROUP-I PROTEIN - ITS CONFORMATION IS NOT AFFECTED BY EXPOSURE TO HIGH-TEMPERATURE

Authors
RUSSOUW PS FARRANT J BRANDT W LINDSEY GG
Citation
Ps. Russouw et al., THE MOST PREVALENT PROTEIN IN A HEAT-TREATED EXTRACT OF PEA (PISUM-SATIVUM) EMBRYOS IS AN LEA GROUP-I PROTEIN - ITS CONFORMATION IS NOT AFFECTED BY EXPOSURE TO HIGH-TEMPERATURE, Seed science research, 7(2), 1997, pp. 117-123
Citations number
26
Categorie Soggetti
Plant Sciences
Journal title
Seed science research → ACNP
ISSN journal
09602585
Volume
7
Issue
2
Year of publication
1997
Pages
117 - 123
Database
ISI
SICI code
0960-2585(1997)7:2<117:TMPPIA>2.0.ZU;2-O
Abstract
The LEA-like protein previously isolated from a homogenate of pea (Pis um sativum L.) embryonic axes heated at 80 degrees C for 10 min (Russo uw et al., 1995) was purified without exposure to heat. Peptides produ ced by trypsin digestion were separated by HPLC and sequenced. The pro tein was identified as a member of the LEA group I family. The conform ation of the protein was compared before and after heat treatment by a ntibody affinity, circular dichroism spectroscopy, fluorescence spectr oscopy and 8-anilino-1-naphthalenesulfonic acid binding. No difference s could be detected, demonstrating that the protein was not irreversib ly denatured by exposure to high temperature.