Molecular cloning and characterization of three novel cytochrome P450 2D isoforms, CYP2D20, CYP2D27, and CYP2D28 in the Syrian hamster (Mesocricetus auratus)

We cloned three novel cytochrome P450 (CYP) 2D cDNAs in the Syrian hamster (Mesocricetus auratus). Each clone contained an open reading frame of 1500 nucleotides encoding a protein of 500 amino acids. The deduced amino acid s equences of these had high identities with those of the other CYP2D members , therefore, the clones were assigned as CYP2D20, CYP2D27, and CYP2D28. Nor thern blot analysis showed that the CYP2D27 mRNA was expressed in liver, bu t not in kidney, small intestine, and brain, while the CYP2D20 and CYP2D28 mRNAs were not detected in these tissues examined. The expression of CYP2D2 7 mRNA in liver did not show sex difference and was not induced by either 3 -methylcholanthrene or phenobarbital treatment. We characterized the enzyme activities of recombinant CYP2D27 expressed in COS-7 cells. The CYP2D27 pr otein had the bufuralol 1'-hydroxylase and debrisoquine 4-hydroxylase activ ities that are specific to the CYP2D subfamily. (C) 2000 Elsevier Science I nc. All rights reserved.