The Vancomycin biosynthetic pathway is induced in oxygen-limited Amycolatopsis orientalis (ATCC 19795) cultures that do not produce antibiotic

Authors
Dunstan, GH Avignone-Rossa, C Langley, D Bushell, ME
Citation
Gh. Dunstan et al., The Vancomycin biosynthetic pathway is induced in oxygen-limited Amycolatopsis orientalis (ATCC 19795) cultures that do not produce antibiotic, ENZYME MICR, 27(7), 2000, pp. 502-510
Citations number
45
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
ENZYME AND MICROBIAL TECHNOLOGY
ISSN journal
01410229 → ACNP
Volume
27
Issue
7
Year of publication
2000
Pages
502 - 510
Database
ISI
SICI code
0141-0229(200010)27:7<502:TVBPII>2.0.ZU;2-O
Abstract
Vancomycin biosynthetic capacity was stimulated in oxygen-limited bioreacto r culture (6.4% DOT) of Amycolatopsis orientalis, coinciding with a down-re gulation of the culture growth and protein synthesis rates. However no Vanc omycin was detected. ATP production was similar in oxygen-limited and oxyge n-sufficient cultures (400-150 mu mole(ATP). g(biomass)(-1)). Our findings suggest a critical requirement for oxygen flux through the Vancomycin biosy nthetic pathway, which is only met under nutrient-limited conditions in whi ch oxygen is in excess. (C) 2000 Elsevier Science Inc. All rights reserved.