A. Muller et al., Detection of Isospora belli by polymerase chain reaction using primers based on small-subunit ribosomal RNA sequences, EUR J CL M, 19(8), 2000, pp. 631-634
Citations number
15
Categorie Soggetti
Microbiology
Journal title
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
The aim of the present study was to use small-subunit (SSU)-rRNA sequences
of Isospora belli to design specific primer pairs and a hybridization probe
for the detection of Isospora belli in human samples by PCR and Southern b
lot hybridization. PCR amplification with the primer pairs produced correct
DNA fragments with target DNA from samples of Isospora belli-infected pati
ents and from cloned SSU-rRNA of Isospora belli. The nature of the PCR prod
ucts was confirmed by Southern blot hybridization. No amplification was see
n with template DNA extracted from other parasites. Although Isospora belli
infections can be easily diagnosed using light microscopy, molecular-based
techniques may prove useful as an additional diagnostic tool.