Synthetically prepared Amadori-glycated phosphatidylethanolamine call trigger lipid peroxidation via free radical reactions

This study for the first time confirmed the peroxidative role of the Amador i product derived from the glycation of phosphatidylethanolamine (PE), name ly Amadori-PE. The product was synthesized from the reaction of dioleoyl PE with D-glucose, and then purified by a solid-phase extraction procedure, w hich was a key step in the next HPLC technique for the isolation of essenti ally pure Amadori-PE. When the synthetically prepared Amadori-PE was incuba ted with linoleic acid in the presence of Fe3+ in micellar system, a remark able formation of thiobarbituric acid reactive substances was observed toge ther with increases in lipid hydroperoxides. In addition, the lipid peroxid ation caused by Amadori-PE nas effectively inhibited by superoxide dismutas e, mannitol, catalase and metal chelator. These results indicated that Amad ori-PE triggers oxidative modification of lipids via the generation of supe roxide, and implied the involvement of 'lipid glycation' along with membran e lipid peroxidation in the pathogenesis of diabetes and aging. (C) 2000 Fe deration of European Biochemical Societies, Published by Elsevier Science B .V. All rights reserved.