A novel human cytochrome P4504F isoform (CYP4F11): cDNA cloning, expression, and genomic structural characterization

By a combination of cDNA library screening and rapid amplification of cDNA ends analysis, a novel human cytochrome P450 4F isoform has been cloned and sequenced. The new 4F isoform is designated CYP4F11 and contains 1765 nucl eotides. The coding region encodes 524 amino acid residues, and the heme-bi nding region is highly conserved. The CYP4F11 amino acid sequence has 80.0, 82.3, and 79.2% identity to CYP4F2, CYP4F3, and CYP4F8 amino acid sequence s, respectively. In vitro translation shows the molecular mass of CYP4F11 i s approximately 57 kDa, consistent with the calculated molecular mass. CYP4 F11 is expressed mainly in human liver, followed by kidney, heart, and skel etal muscle. The genomic structure of CYP4F11 was solved by database search ing and computer analysis. The coding region of CYP4F11 has 12 exons. The C YP4F11 gene is located 16 kb upstream of the CYP4F2 gene on chromosome 19. This is consistent with the notion that the human cytochrome P450 4F genes form a cluster on chromosome 19. (C) 2000 Academic Press.