By analysing a large set of St. Santin F region Incoherent Scatter (IS) dat
a for low solar activity we find nighttime ion temperature (T-i) on average
to be apparently slightly higher than electron temperature (T-e). We show
that this difference is a bias incurred by the IS spectral analysis procedu
re owing to the neglect of H+ ions in the 300-500 km altitude region. Furth
er, we show T-e and T-i to depart from the neutral temperature T-n with inc
reasing altitude. We perform a simulation of ion and electron temperatures
and show that the difference between T-i, T-e and T-n can be caused by the
presence of a small amount of hat oxygen. Using our simulation we estimate
the ratio of hot oxygen to cold oxygen density to be 0.13% on average at 40
0-km altitude for low solar activity.