MHC diversity in Caucasians, investigated using highly heterogeneous noncoding sequence motifs at the DQB1 locus including a retroviral long terminalrepeat element, and its comparison to nonhuman primate homologues

Citation
H. Donner et al., MHC diversity in Caucasians, investigated using highly heterogeneous noncoding sequence motifs at the DQB1 locus including a retroviral long terminalrepeat element, and its comparison to nonhuman primate homologues, IMMUNOGENET, 51(11), 2000, pp. 898-904
Citations number
23
Categorie Soggetti
Immunology
Journal title
IMMUNOGENETICS
ISSN journal
00937711 → ACNP
Volume
51
Issue
11
Year of publication
2000
Pages
898 - 904
Database
ISI
SICI code
0093-7711(200009)51:11<898:MDICIU>2.0.ZU;2-6
Abstract
Long terminal repeats (LTRs) are common retrovirus-related sequences spread throughout the human genome. We previously reported the human-specific int egration of one LTR (DQLTR3) located 15 kb upstream of HLA DQB1. To elucida te the contribution of retroviral sequences to the variability and phylogen etic background of HLA DQB1 we investigated another LTR (DQLTR13), located 1.3 kb upstream of HLA DQB1, in German families, great apes, and Old World monkeys. Within German families, DQLTR13 presence was strongly linked to HL A DQB1"0302, *0303, and *0402 haplotypes. All other haplotypes had a low fr equency or were devoid of DQLTR13. Phylogenetic analysis of DQLTR13 and adj acent nucleotide sequences in humans and nonhuman primates revealed a high degree of similarity and recent origin of HLA DeB1"0302, *0303, and *0402. Nevertheless, two lineages leading to DQB1*0301 and *0302 were generated by an ancient split of a DQB1*0301, *0302 progenitor. A third lineage consist ing of DQB1*05/*06-related sequences may have evolved from the DeB1"0302 li neage, and a DQB1*0201-related sequence shared common ancestry with DQB1*03 01. Among the human haplotypes, HLA DQB1*0201 and *0301 are linked to two d ifferent DQA1 alleles. Based on the small genetic distance of DQLTR13 as we ll as the adjacent sequences on these haplotypes, we suggest that a recent recombination is responsible for these associations. In the analysis of non human primate species, we detected DQLTR13 in two lowland gorillas, dating the integration at at least 8 million years ago. We therefore conclude that noncoding sequences up to 1.3 kb upstream of DQB1 provide novel insight in to the generation of MHC gene diversity.