Ka. Hoag et al., A quantitative-trait locus controlling peripheral B-cell deficiency maps to mouse Chromosome 15, IMMUNOGENET, 51(11), 2000, pp. 924-929
Peripheral B-lymphocyte homeostasis is. determined through incompletely def
ined positive and negative regulatory processes. The A/WySnJ mouse, but not
the related A/J strain, has disturbed homeostasis leading to peripheral B-
lymphocyte deficiency. B lymphopoeisis is normal in A/WySnJ mice, but the B
cells apoptose rapidly in the periphery. This B cell-in-trinsic defect seg
regated as a single locus, Bcmd. in (A/WySnJxA/J)F2 mice. Here we mapped a
quantitative-trait locus (QTL) that contributes to the A/WySnJ B-cell defic
iency by examining the F2 progeny of a cross between strains A/WySnJ and CA
ST/Ei. In this cross, minimally 1.9 QTLs controlling peripheral B lymphocyt
e deficiency segregated. The (A/WySnJxCAST/Ei)F2 mice were phenotyped for s
plenic B-cell percentage and the DNA from progeny with extreme phenotypes w
as used to map the QTL by the simple-sequence length polymorphism method. A
genome scan showed linkage between peripheral B-cell deficiency and Chromo
some (Chr) 15 markers. When closely spaced Chr 15 markers were analyzed, th
e 99% confidence interval for the QTL map position extended along the entir
e chromosome length. The peak lod scores >17 occured between 30 and 45 cM.
We conclude that a significant QTL segregating in (A/WySnJxCAST/Ei)F2 mice
resides in this middle region of Chr 15.