Adsorptive immobilization of erythrocyte membrane

Immobilization of human erythrocyte membrane was carried out by adsorption on Fractosil, a porous form of silica. Acetylcholinesterase (AChE) was chos en as a representative membrane enzyme in this study. Dependency of adsorpt ion on membrane concentration was determined. Positive cooperative interact ions that occurred in the process of immobilization increased stability. Pr esence of hydrophobic ligands on derivatized Fractosil was found to enhance stability of immobilized preparations making them more effective for use i n continuous catalytic transformations. It is suggested that adsorptive imm obilization of membrane structures such as the human erythrocyte membrane f ragments on Fractosil and other inexpensive supports may provide a convenie nt procedure for utilization of their catalytic potential. Such preparation s may be used in diagnostic kits or for construction of biosensors. (C) 200 0 Elsevier Science B.V. All rights reserved.