Jd. Wood et al., Atrophin-1, the dentato-rubral and pallido-luysian atrophy gene product, interacts with ETO/MTG8 in the nuclear matrix and represses transcription, J CELL BIOL, 150(5), 2000, pp. 939-948
Dentato-rubral and pallido-luysian atrophy (DRPLA) is one of the family of
neurodegenerative diseases caused by expansion of a polyglutamine tract. Th
e drp1a gene product, atrophin-1, is widely expressed, has no known functio
n or activity, and is found in both the nuclear and cytoplasmic compartment
s of neurons. Truncated fragments of atrophin-1 accumulate in neuronal nucl
ei in a transgenic mouse model of DRPLA, and may underlie the disease pheno
type.
Using the yeast two-hybrid system, we identified ETO/MTG8, a component of n
uclear receptor corepressor complexes, as an atrophin-1-interacting protein
. When cotransfected into Neuro-2a cells, atrophin-1 and ETO/MTG8 colocaliz
e in discrete nuclear structures that contain endogenous mSin3A and histone
deacetylases. These structures are sodium dodecyl sulfate-soluble and asso
ciated with the nuclear matrix. Cotransfection of ETO/MTG8 with atrophin-1
recruits atrophin-1 to the nuclear matrix, while atrophin-1 and ETO/MTG8 co
fractionate in nuclear matrix preparations from brains of DRPLA transgenic
mice. Furthermore, in a cell transfection-based assay, atrophin-1 represses
transcription. Together, these results suggest that atrophin-1 associates
with nuclear receptor corepressor complexes and is involved in transcriptio
nal regulation.
Emerging links between disease-associated polyglutamine proteins, nuclear r
eceptors, translocation-leukemia proteins, and the nuclear matrix may have
important repercussions for the pathobiology of this family of neurodegener
ative disorders.