Use of amino acid analysis for estimating the individual concentrations ofproteins in mixtures

The concentrations of five individual proteins in a mixture were determined from one amino acid analysis of the mixture by solving for each protein us ing simultaneous equations. Dried casein and whey were separated into five individual protein components using reversed-phase HPLC. Individual protein s were collected and analyzed for amino acid composition. These data were u sed as standards. Mixtures of purified proteins were analyzed for total ami no acid composition and the concentrations of individual proteins in the mi xtures were determined by solving simultaneous equations based on the amino acid analysis composition of the standards. (C) 2000 Elsevier Science B.V. All rights reserved.