OPTIMIZED METHODS FOR CAPILLARY ELECTROPHORESIS OF TETRACYCLINES

Optimized methods for the analysis of some tetracyclines by capillary electrophoresis are described. Different buffer systems were employed for the separation of tetracycline, oxytetracycline and demeclocycline from their respective major impurities, including the 2-acetyl-2-deca rboxamido derivatives. The influence of buffer pH and buffer concentra tion was systematically investigated. Non-ionic surfactant Triton X-10 0 and methyl-beta-cyclodextrin were used to obtain improved selectivit y in the case of oxytetracycline and demeclocycline. The results are c ompared with those of previously established liquid chromatography met hods. Good correlations were obtained. (C) 1997 Elsevier Science B.V.