Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor

Authors
Park, LS Martin, U Garka, K Gliniak, B Di Santo, JP Muller, W Largaespada, DA Copeland, NG Jenkins, NA Farr, AG Ziegler, SF Morrissey, PJ Paxton, R Sims, JE
Citation
Ls. Park et al., Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor, J EXP MED, 192(5), 2000, pp. 659-669
Citations number
37
Categorie Soggetti
Medical Research General Topics
Journal title
JOURNAL OF EXPERIMENTAL MEDICINE
ISSN journal
00221007 → ACNP
Volume
192
Issue
5
Year of publication
2000
Pages
659 - 669
Database
ISI
SICI code
0022-1007(20000904)192:5<659:COTMTS>2.0.ZU;2-V
Abstract
The,cellular receptor for murine thymic stromal lymphopoietin (TSLP) was de tected in a variety of murine, but not human myelomonocytic cell Lines by r adioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)R alpha ch ain cDNA resulted in conversion to high affinity binding. TSLP did not acti vate cells from IL7R alpha(-/-) mice, but did activate cells h-om gamma c(- /-) mice. Thus, the functional TSLPR requires the IL-7R alpha chain, but no t the gamma c chain for signaling.