Development of a biofilm formation method for waste forms stability evaluation

The development of an accurate assessment protocol is critical for the pred iction of long-term performance of waste disposal systems under field condi tions. In this study, the development of a biofilm formation method for the evaluation of waste forms stability to microbially induced degradation (MI D) is reported. The development process involved significant modifications to the existing Nuclear Regulatory Commission (NRC) approach, In the biofil m formation method, the control media and fermenter broths are designed to be of similar pH to avoid overestimation of the microbe's capability to deg rade the waste forms. In the NRC approach, the pH values are different, The existing one-stage process of the NRC approach is also replaced with a two -stage process in the biofilm formation method. This is to ensure full eval uation of the microbe's involvement in waste forms degradation. The first s tage of the two-stage process is for biofilm formation and the second is fo r biofilm evaluation. The use of a two-stage process eliminates the possibi lity of substrate limitation, resulting in values of degradation indices th at are about two times higher than those obtained using the single-stage NR C approach. Two waste forms (100% Tuskegee cement and 21% cobalt chloride/7 9% cement) were used in the development of the biofilm formation method. Bo th waste forms showed evidence of biofilm formation. The formation of biofi lm on the cobalt-containing waste form indicates a lack of anti-microbial c apability of cobalt. (C) 2000 Elsevier Science B.V. All rights reserved.