Regeneration of PAPS for the enzymatic synthesis of sulfated oligosaccharides

This paper describes the study of 3'-phosphoadenosine-5'-phosphosulfate (PA PS) regeneration from 3'-phosphoadenosine-5'-phosphate (PAP) for use in pra ctical syntheses of carbohydrate sulfates which are catalyzed by sulfotrans ferases. Among the regeneration systems, the one with recombinant aryl sulf otransferase proved to be the most practical. This regeneration system was coupled with a sulfotransferase-catalyzed reaction, using a recombinant Nod factor sulfotransferase, for the synthesis of various oligosaccharide sulf ates that were further glycosylated using glycosyltransferases.