Recognition of DNA topology in reactions between plasmid DNA and cationic copolymers

Citation
Tk. Bronich et al., Recognition of DNA topology in reactions between plasmid DNA and cationic copolymers, J AM CHEM S, 122(35), 2000, pp. 8339-8343
Citations number
17
Categorie Soggetti
Chemistry & Analysis",Chemistry
Journal title
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN journal
00027863 → ACNP
Volume
122
Issue
35
Year of publication
2000
Pages
8339 - 8343
Database
ISI
SICI code
0002-7863(20000906)122:35<8339:RODTIR>2.0.ZU;2-X
Abstract
This study for the first time demonstrates phenomenon of recognition of DNA tertiary structure by the synthetic polycationic molecules. Effects of DNA topology were evaluated using supercoiled and linearized forms of plasmid DNA (scDNA and lDNA). Recognition is achieved by using relatively simple ch emical structures interacting with the DNA. Two polycations modified with w ater-soluble poly(ethylene glycol) (PEG) chains, PEG- block-poly(N-methyl-4 -vinylpyridinium sulfate) (PEC-b-PVP) and PEG -graft-polyethyleneimine (PEG -g-PEI) were used. When added to the mixtures of lDNA and scDNA, PEO-b-PVP selectively bound to scDNA, while lDNA remained free. In contrast, PEO-g-PE I interacted with both forms of the DNA present in the mixture. Distinct be havior of two copolymers was attributed to the differences in their structu re, particularly, charge density of the polycation blocks. Relatively small variation in the polycation ionization state can result in drastic changes in its behavior upon interaction with DNA. Particularly, the change of pH from 7.0 to 5.0, increasing the charge density of PEI block in PEO-g-PEI, w as also accompanied by the appearance of recognition phenomena. These findi ngs uncover possibilities for the control of the processes of DNA incorpora tion in the complexes with cationic species by altering the DNA topology, w hich may have practical significance in using these complexes for gene deli very.