S. Kobal et al., Serum T3 and T4 concentrations in the adult rats treated with herbicide 2,4-dichlorophenoxyacetic acid, PFLUG ARCH, 440(5), 2000, pp. R171-R172
The influence of sublethal doses of 2,4-dichlorophenoxyacetic acid (2,4-D)
on serum T3 and T4 concentrations in Hsd Cpb: Wistar rats of both sexes was
studied. The trial was performed on 24 males and females respectively, eac
h divided into three groups of 8 animals (control, groups 1 and 2). Aqueous
solution of the compound (11 mg/kg body weight - group 1 and 110 mg/kg bod
y weight - group 2) or clean tap water (control group) was used. Aliquots o
f 2.4 ml/kg body weight were administered with a stomach tube from the 1(st
) to 10(th) day of the experiment. Three days before the first treatment an
d on the 6(th) and 13(th) day of the experiment the serum T3 and T4 concent
rations were determined by commercial radioimmunoassay kits (Byk-Sangtec Di
agnostica), validated for rats. A significant decrease of serum T4 (P < 0.0
1) and T3 (P < 0.001) was determined in males of groups 1 and 2 during the
experiment. On the 6(th) day of experiment serum T4 and T3 values were sign
ificantly lower (P < 0.001 and 0.01 respectively) in group 2 than in the co
ntrols and group 1 of both males and females. During the whole experiment s
erum T4 levels were lower in females than in males (P < 0.05).