Characterization of protein and transcript levels of the chaperonin containing tailless complex protein-1 and tubulin during light-regulated growth of oat seedlings

In grass seedlings the network of cortical microtubules is reorganized duri ng light-dependent growth of coleoptiles and mesocotyls. We investigated th e effects of light-dependent growth on the relative steady-state levels of the mRNAs and protein levels of alpha-tubulin and the epsilon-subunit of th e chaperonin containing tailless complex protein-1 in oat (Avena sativa) co leoptiles, which were grown in different light conditions to establish diff erent growth responses. The soluble pools of the epsilon-subunit of the cha peronin containing tailless complex protein-1 and alpha-tubulin decreased i n nonelongating coleoptiles, suggesting that the dynamics of the light-regu lated soluble pool reflect the processes occurring during reorganization of cortical microtubules. The shifts in pool sizes are discussed in relation to the machinery that controls the dynamic structure of cortical microtubul es in plant cells.