A broad-host-range plasmid for isolating mobile genetic elements in gram-negative bacteria

Authors
Schneider, D Faure, D Noirclerc-Savoye, M Barriere, AC Coursange, E Blot, M
Citation
D. Schneider et al., A broad-host-range plasmid for isolating mobile genetic elements in gram-negative bacteria, PLASMID, 44(2), 2000, pp. 201-207
Citations number
28
Categorie Soggetti
Molecular Biology & Genetics
Journal title
PLASMID
ISSN journal
0147619X → ACNP
Volume
44
Issue
2
Year of publication
2000
Pages
201 - 207
Database
ISI
SICI code
0147-619X(200009)44:2<201:ABPFIM>2.0.ZU;2-7
Abstract
Plasmid pGBG1 was constructed to isolate mobile genetic elements in a wide variety of gram-negative bacteria. The mutation target, carried on a broad- host-range vector, allows positive selection for tetracycline resistance. I n tests using several gram-negative bacteria we could detect transposition events of either insertion sequences or transposons. A new insertion sequen ce (IS) element was identified in Ralstonia eutropha. (C) 2000 Academic Pre ss.