CHEMOENZYMATIC ONE-POT-SYNTHESIS OF CEFAZOLIN FROM CEPHALOSPORIN-C INFULLY AQUEOUS-MEDIUM, INVOLVING 3 CONSECUTIVE BIOTRANSFORMATIONS CATALYZED BY D-AMINO-ACID OXIDASE, GLUTARYL ACYLASE AND PENICILLIN-G ACYLASE

Authors
FERNANDEZLAFUENTE R GUISAN JM PREGNOLATO M TERRENI M
Citation
R. Fernandezlafuente et al., CHEMOENZYMATIC ONE-POT-SYNTHESIS OF CEFAZOLIN FROM CEPHALOSPORIN-C INFULLY AQUEOUS-MEDIUM, INVOLVING 3 CONSECUTIVE BIOTRANSFORMATIONS CATALYZED BY D-AMINO-ACID OXIDASE, GLUTARYL ACYLASE AND PENICILLIN-G ACYLASE, Tetrahedron letters, 38(26), 1997, pp. 4693-4696
Citations number
16
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
38
Issue
26
Year of publication
1997
Pages
4693 - 4696
Database
ISI
SICI code
0040-4039(1997)38:26<4693:COOCFC>2.0.ZU;2-O
Abstract
A new chemoenzymatic synthesis of Cefazolin through the correct assemb ly of three biotransformations catalysed by D-aminoacid oxidase, gluta ryl acylase and penicillin G acylase is described. This multienzymatic synthesis has been performed from the natural Cephalosporin C in full y aqueous medium without intermediate purification stages. Almost quan titative yields have been achieved in all the enzymatic reactions. (C) 1997 Elsevier Science Ltd.