Regeneration of foreign genes co-transformed plants of Medicago sativa L by Agrobacterium rhizogenes

Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were tran sferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeci ens. Regeneration of transgenic plants was induced successfully from hairy root tissue of cotyledon in alfalfa. Cotyledon tissues were an ideally tran sformed recipient. There was a negative correlation between age of hairy ro ots and embryogenesis frequency in alfalfa. Production of cc-transformed pl ants with greater yield and super quality was important for development of new alfalfa varieties.