Molecular cloning and expression analysis of HAG1 in the floral organs of Hyacinthus orientalis L.

HAG1 gene was isolated from the floral organs of Hyacinthus orientalis L, b y using RT-PCR. Sequence analysis showed that this gene was homologous to A GAMOUS. Northern hybridization indicated that HAG1 was specifically express ed in floral organs using 3' end of HAG1 as a probe. Further, transcript of this gene was not detected in differentiating tepals induced by lower conc entration of hormones, however, it was detected in differentiating stemans by higher concentration of hormones in vitro. It is possible that there is a close relationship between the concentration of hormones, homeotic genes and identities of floral organs.