Golgi 58K-like protein in pollens and pollen tubes of Lilium davidii

In animal cells, Golgi apparatus is located near the microtubule organizing center (MTOC) and its position is determined partly by 58K protein. By sod ium dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immun e-blotting methods, a 58K-like protein has been found in pollen grains and pollen tubes of Lilium davidii, Its molecular weight is very similar to tha t of the 58K protein of animal cells. By immunofluorescence labeling, under a confocal laser scanning microscope (CLSM), the animal 58K antibody revea led a punctate staining in pollen grains and pollen tubes, which is consist ent with the distribution of Golgi apparatus in plant cells. In addition, i mmune-gold labeling and transmission electron microscopy showed that the 58 K-like protein bound mainly to the membrane of vesicles-like structure near Golgi apparatus, This is the first demonstration of the 58K-like protein i n plant cells.